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pro12a general polarization  (Tecan Systems)


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    Structured Review

    Tecan Systems pro12a general polarization
    a. <t>Pro12A</t> GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.
    Pro12a General Polarization, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 17567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro12a+general+polarization/Spark/bio_rxiv__2024__10__04__616688-394-0-14
    Average 99 stars, based on 17567 article reviews
    pro12a general polarization - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cells"

    Article Title: Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cells

    Journal: bioRxiv

    doi: 10.1101/2024.10.04.616688

    a. Pro12A GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.
    Figure Legend Snippet: a. Pro12A GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.

    Techniques Used: Liposomes, Fluorescence

    a . Membrane lipidome species sorted by abundance from the largest to the lowest, with an average power fit shown as a black line. Dashed vertical lines show the separation point of lipidomes into high- and low- abundance species, using Kneed algorithm (see methods for details). The inset barplots show the average abundance, covered by the high-abundance species in the sample. b . Numberical value for each of the samples average power fit, plotted against the respective lipidome size. c . Average R2 (goodness of fit) plotted against the respective lipidome size for each of the diets. For all scatter plots in a,b and c the markers represent the average value for 6 replicates (3 for 37°C and 3 for 30°C), with respective standard deviations. d . Relative cellular fitness, estimated as the sum of ranks, assigned to each of the experimental readouts in this study (growth rates at 37°C, homeoviscous adaptation as the difference between Pro12A GP values at 37°C and 30°C, osmotic sensitivity at 37°C and 30°C). For details see methods.
    Figure Legend Snippet: a . Membrane lipidome species sorted by abundance from the largest to the lowest, with an average power fit shown as a black line. Dashed vertical lines show the separation point of lipidomes into high- and low- abundance species, using Kneed algorithm (see methods for details). The inset barplots show the average abundance, covered by the high-abundance species in the sample. b . Numberical value for each of the samples average power fit, plotted against the respective lipidome size. c . Average R2 (goodness of fit) plotted against the respective lipidome size for each of the diets. For all scatter plots in a,b and c the markers represent the average value for 6 replicates (3 for 37°C and 3 for 30°C), with respective standard deviations. d . Relative cellular fitness, estimated as the sum of ranks, assigned to each of the experimental readouts in this study (growth rates at 37°C, homeoviscous adaptation as the difference between Pro12A GP values at 37°C and 30°C, osmotic sensitivity at 37°C and 30°C). For details see methods.

    Techniques Used: Membrane

    Related Articles

    Incubation:

    Article Title: Temperature change elicits lipidome adaptation in the simple organisms Mycoplasma mycoides and JCVI-syn3B.
    Article Snippet: .. Themeasurement plate with labelled bacterial samples is incubated at a growth temperature for 20 min, fluorescence recorded at 440 nm and 490 nm, with 20 nm bandwidth, following excitation at 360 nm +/ 10 nm Pro12A general polarization was calculated according to the formula Pro12A GP = I440 I490 I440 +I490 Measurements are carried out in Tecan Spark 20multimodemicroplate reader, equipped with a thermostat capable of maintaining the temperature with the accuracy of +/ 1 C. To ensure measurement reproducibility, restraining the harvesting window of cellular growth state is crucial. .. All consumables, wash buffer, Tecan Spark and the centrifuge are preheated to the bacterial growth temperature for at least 1 h prior themeasurement.

    Fluorescence:

    Article Title: Temperature change elicits lipidome adaptation in the simple organisms Mycoplasma mycoides and JCVI-syn3B.
    Article Snippet: .. Themeasurement plate with labelled bacterial samples is incubated at a growth temperature for 20 min, fluorescence recorded at 440 nm and 490 nm, with 20 nm bandwidth, following excitation at 360 nm +/ 10 nm Pro12A general polarization was calculated according to the formula Pro12A GP = I440 I490 I440 +I490 Measurements are carried out in Tecan Spark 20multimodemicroplate reader, equipped with a thermostat capable of maintaining the temperature with the accuracy of +/ 1 C. To ensure measurement reproducibility, restraining the harvesting window of cellular growth state is crucial. .. All consumables, wash buffer, Tecan Spark and the centrifuge are preheated to the bacterial growth temperature for at least 1 h prior themeasurement.



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    pro12a general polarization  (Tecan Systems)
    99
    Tecan Systems pro12a general polarization
    a. <t>Pro12A</t> GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.
    Pro12a General Polarization, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro12a+general+polarization/Spark/bio_rxiv__2024__10__04__616688-394-0-14
    Average 99 stars, based on 1 article reviews
    pro12a general polarization - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    a. Pro12A GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.

    Journal: bioRxiv

    Article Title: Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cells

    doi: 10.1101/2024.10.04.616688

    Figure Lengend Snippet: a. Pro12A GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.

    Article Snippet: Pro12A general polarization was calculated according to the formula: Measurements are carried out in Tecan Spark 20 multimode microplate reader, equipped with a thermostat capable of maintaining the temperature with the accuracy of +/- 1°C.

    Techniques: Liposomes, Fluorescence

    a . Membrane lipidome species sorted by abundance from the largest to the lowest, with an average power fit shown as a black line. Dashed vertical lines show the separation point of lipidomes into high- and low- abundance species, using Kneed algorithm (see methods for details). The inset barplots show the average abundance, covered by the high-abundance species in the sample. b . Numberical value for each of the samples average power fit, plotted against the respective lipidome size. c . Average R2 (goodness of fit) plotted against the respective lipidome size for each of the diets. For all scatter plots in a,b and c the markers represent the average value for 6 replicates (3 for 37°C and 3 for 30°C), with respective standard deviations. d . Relative cellular fitness, estimated as the sum of ranks, assigned to each of the experimental readouts in this study (growth rates at 37°C, homeoviscous adaptation as the difference between Pro12A GP values at 37°C and 30°C, osmotic sensitivity at 37°C and 30°C). For details see methods.

    Journal: bioRxiv

    Article Title: Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cells

    doi: 10.1101/2024.10.04.616688

    Figure Lengend Snippet: a . Membrane lipidome species sorted by abundance from the largest to the lowest, with an average power fit shown as a black line. Dashed vertical lines show the separation point of lipidomes into high- and low- abundance species, using Kneed algorithm (see methods for details). The inset barplots show the average abundance, covered by the high-abundance species in the sample. b . Numberical value for each of the samples average power fit, plotted against the respective lipidome size. c . Average R2 (goodness of fit) plotted against the respective lipidome size for each of the diets. For all scatter plots in a,b and c the markers represent the average value for 6 replicates (3 for 37°C and 3 for 30°C), with respective standard deviations. d . Relative cellular fitness, estimated as the sum of ranks, assigned to each of the experimental readouts in this study (growth rates at 37°C, homeoviscous adaptation as the difference between Pro12A GP values at 37°C and 30°C, osmotic sensitivity at 37°C and 30°C). For details see methods.

    Article Snippet: Pro12A general polarization was calculated according to the formula: Measurements are carried out in Tecan Spark 20 multimode microplate reader, equipped with a thermostat capable of maintaining the temperature with the accuracy of +/- 1°C.

    Techniques: Membrane